企業(yè)檔案

Product Name	c-Myb Antibody (M-19)
Antibody Type	Primary Antibodies
Modification Notes	The highly leukemogenic avian retrovirus E26 contains two oncogenes, v-Myb ａnd v-Ets, which are expressed together as a fusion protein. The cellular homolog of v-Myb, designated c-Myb, encodes a transcription factor. Deletion or disruption of a negative regulatory domain mapping within the carboxy terminal domain of c-Myb results in enhanced transactivating capacity ａnd in parallel, leads to activation of its ability to transform hemopoietic cells. c-Myb is expressed preferentially, but not exclusively, in immature hemopoietic cells ａnd its expression decreases as cells differentiate. A second member of the Myb proto-oncogene family, B-Myb, encodes a second sequence-specific DNA binding protein. B-Myb RNA levels are low or undetectable in quiescent cells but increase at the G1 to S phase transition following mitogenic stimulation. Studies suggest that B-Myb expression rescues cells from p53-induced G1 arrest mediated by p21.

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	WBIPIFELISAGSChIP
	recommended for detection of c-Myb of mouse, rat and, to a lesser extent, human origin by WB, IP, IF ａnd ELISA; also reactive with additional species, including equine, canine ａnd porcine:
Species Reactivity	HumanMouseRat
Concentration	1mg/ml
Purification	Affinity purified

Alternative Names	epie mapping at the C-terminus of c-Myb of mouse origin
Tissue Specificity	epie mapping at the C-terminus of c-Myb of mouse origin

Entrez Gene	4602

Application

Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear ａnd cylasmic localization.
Application

Western blot analysis of c-Myb expression in non-transfected: (A) ａnd mouse c-Myb transfected: (B) 293T whole cell lysates.
Application

Western blot analysis of c-Myb expression in Jurkat whole cell lysate.
Application

Western blot analysis of c-Myb expression in non-transfected: (A) ａnd human c-Myb transfected: (B) 293T whole cell lysates.
Application

Western blot analysis of c-Myb expression in F9 whole cell lysate.

Application Notes	recommended for detection of c-Myb of mouse, rat and, to a lesser extent, human origin by WB, IP, IF ａnd ELISA; also reactive with additional species, including equine, canine ａnd porcine:

Form	Liquid
Storage Instructions	For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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