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技術(shù)文章

PINCH antibody Catalog Number：OM268604

點(diǎn)擊次數(shù)：30 發(fā)布時(shí)間：2024/9/29 14:12:14
 

Product Profile

Product Name PINCH antibody
Antibody Type Primary Antibodies
Product description 
Mouse Monoclonal Antibody [5G7] to PINCHThe protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
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Immunogen 
The immunogen corresponding to human PINCH expressed in E. Coli.

Key Feature

Clonality Monoclonal
Isotype IgG1
Clone Number 5G7
Host Species Mouse
Tested Applications

ELISAFACSICC/IFWB
 
ELISA: 1/10000.:
Species Reactivity

Human
Concentration 1 mg/ml
Purification  

Target Information

Gene Synonyms
PINCH-1
LIMS1
PINCH1
Target PINCH
Molecular Weight(MW) 37 kDa (note)

Application

  • Application

    ICC/IF analysis of HepG2 cells using PINCH antibody [5G7] (green), Alexa Fluor-555 phalloidin (red) ａnd DRAQ5 fluorescent DNA dye (blue).

  • Application

    WB analysis of (1) A549, (2) Jurkat ａnd (3) HeLa lysates using PINCH antibody [5G7].

  • Application

    WB analysis of human PINCH (a.a. 87-249) recombinant protein using PINCH antibody [5G7].

  • Application

    ELISA analysis of control antigen ａnd antigen using PINCH antibody [5G7].

  • Application

    FACS analysis of Hela cells using PINCH antibody [5G7] (blue) or a negative control (red).

Application Notes
ELISA: 1/10000.:

Additional Information

Form Liquid
Storage Instructions 4°C; -20°C for long term storage
Storage Buffer Ascitic fluid containing 0.03% sodium azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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