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技術文章

PINCH [B9-G7] Catalog Number：OM627765

點擊次數(shù)：26 發(fā)布時間：2024/9/29 14:11:14
 

Product Profile

Product Name PINCH [B9-G7]
Antibody Type Primary Antibodies
Product description 
Pinch, also designated particularly interesting new Cys-His protein or NY-REN-48, is a focal adhesion protein that is a component of the ILK-PINCH complex. This complex is a major part of the growth factor ａnd integrin signaling pathway. Pinch is involved in cell differentiation, proliferation ａnd survival by acting as an effector of integrin ａnd growth factor signaling. It is a cylasmic protein expressed in most tissues ａnd consists of five LIM domains, a nuclear localization signal ａnd a nuclear export signal. The PINCH-1/ILK complex is regulated by a PINCH-1 related protein PINCH-2, which also forms a complex with ILK.
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Immunogen 
Recombinant protein

Key Feature

Clonality Monoclonal
Isotype IgG1
Host Species Mouse
Tested Applications

WBICCFC
 
WB:1:500-1:1,000
ICC:1:100-1:500
FC:1:100-1:200
Species Reactivity

Human
Concentration 2 mg/mL.

Target Information

Alternative Names
2310016J22Rik antibody 4921524A02Rik antibody AI507642 antibody AU021743 antibody AW551584 antibody C430041B13Rik antibody LIM ａnd senescent cell antigen like domains 1 antibody LIM ａnd senescent cell antigen-like-containing domain protein 1 antibody LIM zinc finger domain containing 1 antibody LIM-type zinc finger domains 1 antibody Lims1 antibody LIMS1_HUMAN antibody Lims1l antibody Particularly interesting new Cys His protein 1 antibody Particularly interesting new Cys His protein antibody Particularly interesting new Cys-His protein 1 antibody PINCH 1 antibody PINCH antibody PINCH-1 antibody PINCH1 antibody Renal carcinoma antigen NY REN 48 antibody Renal carcinoma antigen NY-REN-48 antibody senescent cell antigen antibody wu:fc32c03 antibody zgc:112533 antibody
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Molecular Weight(MW) 37 kDa
Cellular Localization Cell junction.

Database Links

SwissProt ID P48059

Application

  • Application

    Fig1: Western blot analysis of PINCH on human PINCH recombinant protein using anti-PINCH antibody at 1/1,000 dilution.

  • Application

    Fig2: Western blot analysis of PINCH on different cell lysates using anti-PINCH antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: Jurkat Lane 3: Hela

  • Application

    Fig3: ICC staining PINCH (green) ａnd actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: Flow cytometric analysis of Hela cells with PINCH antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).

Positive Control Human PINCH recombinant protein, A549, Jurkat, Hela, HepG2.
Application Notes
WB:1:500-1:1,000
ICC:1:100-1:500
FC:1:100-1:200

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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