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聯(lián)系人:楊燕燕
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技術文章
PINCH Catalog Number:OM640950
Product Profile
Product Name | PINCH |
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Antibody Type | Primary Antibodies |
Immunogen | Purified recombinant fragment of human PINCH expressed in E. Coli. |
Key Feature
Clonality | Monoclonal |
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Host Species | Unknow |
Tested Applications | WBICCFCMICC/IF |
WB:1:250~1:1000 More ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
Species Reactivity | Human |
Target Information
Alternative Names | More |
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Molecular Weight(MW) | 37kDa |
Database Links
Entrez Gene | 3987 |
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Application
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Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
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Application
Figure 1: Western blot analysis using PINCH mAb against human PINCH (AA: 87-249) recombinant protein. (Expected MW is 44.2 kDa)
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Application
Figure 2: Western blot analysis using PINCH mouse mAb against A549 (1), Jurkat (2), and Hela (3) cell lysate.
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Application
Figure 3: Immunofluorescence analysis of HepG2 cells using PINCH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Application
Figure 4: Flow cytometric analysis of Hela cells using PINCH mouse mAb (blue) and negative control (red).
Application Notes | WB:1:250~1:1000 More ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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Additional Information
Form | Ascitic fluid containing 0.03% sodium azide. |
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Storage Instructions | 4℃; -20℃ for long term storage |
原創(chuàng)作者:上海篤瑪生物科技有限公司