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技術(shù)文章

ITK antibody , C-term Catalog Number：OM260060

點(diǎn)擊次數(shù)：25 發(fā)布時(shí)間：2024/9/29 13:59:36
 

Product Profile

Product Name ITK antibody , C-term
Antibody Type Primary Antibodies
Product description 
Rabbit polyclonal antibody to ITK (IL2-inducible T-cell kinase)This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 ａnd SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation ａnd differentiation. [provided by RefSeq]
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Immunogen 
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ITK. The exact sequence is proprietary.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ICC/IFWB
 
*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity

Human
Predicted Reactivity Rhesus MonkeyRhesus Monkey (100%)
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
EMT antibody
LYK antibody
MGC126257 antibody
MGC126258 antibody
PSCTK2 antibody
ITK antibody
kinase EMT antibody
tyrosine-protein kinase LYK antibody
T-cell-specific kinase antibody
tyrosine-protein kinase ITK/TSK antibody
homolog of mouse T-cell
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Target ITK
Molecular Weight(MW) 72 kDa (note)
Cellular Localization Cell membrane (By similarity)

Application

  • Application

    Sample(30 μg of whole cell lysate) A:293T B:H1299 7.5% SDS PAGE diluted at 1:1500

  • Application

    ITK antibody [C3], C-term detects ITK protein at cylasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ITK protein stained by ITK antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.

Positive Control 293T , H1299
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher. :

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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