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ITK [A6-G12] Catalog Number：OM627718

點擊次數(shù)：26 發(fā)布時間：2024/9/29 13:58:10
 

Product Profile

Product Name ITK [A6-G12]
Antibody Type Primary Antibodies
Product description 
The Tec family of non-receptor tyrosine kinases is composed of six proteins designated Tec, Emt (also known as Itk or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) ａnd Dsrc28C. Four alternatively spliced forms of Tec are found to be expressed broadly in cells Emt gene product associates with CD28 ａnd becomes activated subsequent to CD28 ligation. Btk is necessary for proper B cell development, ａnd mutations in the gene encoding Btk have been associated with families suffering from X-linked agammaglobulinemia, also referred to as Bruton＇s disease. The Bmx protein shares a high degree of homology with Btk ａnd seems to be expressed at highest levels in the heart. Txk expression is T cell-specific, while expression of the Drosophila Tec homolog, Dsrc28C, is developmentally regulated.
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Immunogen 
Recombinant protein

Key Feature

Clonality Monoclonal
Isotype IgG1
Host Species Mouse
Tested Applications

WBICCFC
 
WB:1:500-1:1,000
ICC:1:50-1:200
FC:1:100-1:200
Species Reactivity

Human
Concentration 2 mg/mL.

Target Information

Alternative Names
EMT antibody Homolog of mouse T cell itk/tsk antibody IL 2 inducible T cell kinase antibody IL2 inducible T cell kinase antibody Interleukin 2 inducible T cell kinase antibody Itk antibody ITK_HUMAN antibody Kinase EMT antibody LPFS1 antibody LYK antibody MGC126257 antibody MGC126258 antibody PSCTK 2 antibody PSCTK2 antibody T cell specific kinase antibody T-cell-specific kinase antibody TSK antibody Tyrosine protein kinase antibody Tyrosine protein kinase ITK/TSK antibody Tyrosine protein kinase Lyk antibody Tyrosine-protein kinase ITK/TSK antibody Tyrosine-protein kinase Lyk antibody
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Molecular Weight(MW) 72 kDa
Cellular Localization Cylasm.

Database Links

SwissProt ID Q08881

Application

  • Application

    Fig1: Western blot analysis of ITK on human ITK recombinant protein using anti-ITK antibody at 1/1,000 dilution.

  • Application

    Fig2: Western blot analysis of ITK on Jurkat cell lysate using anti-ITK antibody at 1/1,000 dilution.

  • Application

    Fig3: ICC staining ITK (green) in Jurkat cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: Flow cytometric analysis of Jurkat cells with ITK antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).

Positive Control Human ITK recombinant protein, Jurkat.
Application Notes
WB:1:500-1:1,000
ICC:1:50-1:200
FC:1:100-1:200

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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