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技術文章

PSMB8 antibody Catalog Number：OM272721

點擊次數(shù)：49 發(fā)布時間：2024/9/27 15:08:23
 

Product Profile

Product Name PSMB8 antibody
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to PSMB8The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits ａnd 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration ａnd cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon ａnd this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative t
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Immunogen 
Recombinant protein of human PSMB8

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ICC/IFIHCIHC-PWB
 
WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200:
Species Reactivity

HumanMouseRat
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Synonyms
D6S216E
RING10
ALDD
LMP7
D6S216
JMP
proteasome subunit beta 8
PSMB5i
NKJO
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Target PSMB8

Application

  • Application

    Western blot analysis of extracts of various cell lines, using PSMB8 antibody.

  • Application

    Immunohistochemistry of paraffin-embedded rat heart using PSMB8 antibody at dilution of 1:100 (x400 lens).

  • Application

    Immunohistochemistry of paraffin-embedded human colon using PSMB8 antibody at dilution of 1:100 (x400 lens).

  • Application

    Immunohistochemistry of paraffin-embedded human kidney using PSMB8 antibody at dilution of 1:100 (x400 lens).

  • Application

    Immunofluorescence analysis of A549 cell using PSMB8 antibody. Blue: DAPI for nuclear staining.

Application Notes
WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200:

Additional Information

Form Liquid
Storage Instructions Store at -20℃. Avoid freeze / thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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