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技術文章

MCM7 antibody , Internal Catalog Number：OM262674

點擊次數(shù)：38 發(fā)布時間：2024/9/27 14:58:36
 

Product Profile

Product Name MCM7 antibody , Internal
Antibody Type Primary Antibodies
Product description 
Rabbit polyclonal antibody to MCM7 (minichromosome maintenance complex component 7)The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) ａnd may be involved in the formation of replication forks ａnd in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein ａnd MCM2, 4 ａnd 6 proteins possesses DNA helicase activity, ａnd may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, ａnd may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]
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Immunogen 
Recombinant protein encompassing a sequence within the center region of human MCM7. The exact sequence is proprietary.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ICC/IFIHC-PIPWBChIP
 
*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity

Human
Predicted Reactivity Mouse, Rat, Zebrafish, Xenopus laevis, BovineMouse (92%), Rat (93%), Zebrafish (85%), Xenopus laevis (87%), Bovine (98%)
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Synonyms
CDC47 antibody
MCM2 antibody
P1.1-MCM3 antibody
P1CDC47 antibody
P85MCM antibody
PNAS146 antibody
MCM7 antibody
homolog of S. cerevisiae Cdc47 antibody
minichromosome maintenance deficient 7 antibody
CDC47 homolog antibody
DNA replication licensi
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Target MCM7
Molecular Weight(MW) 81 kDa (note) (3)
Cellular Localization Nucleus

Application

  • Application

    MCM7 antibody [N2C2], Internal immunoprecipitates MCM7 protein in IP experiments. IP samples: HeLa whole cell extract A. 40 μg HeLa whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of MCM7 protein by 4 μg MCM7 antibody [N2C2], Internal 7.5 % SDS-PAGE The immunoprecipitated MCM7 protein was detected by MCM7 antibody [N2C2], Internal diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

  • Application

    Cross-linked ChIP was performed with HeLa chromatin extract ａnd 5 μg of either control rabbit IgG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.

  • Application

    Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM7 antibody at 1:200 dilution.

  • Application

    Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using MCM7 antibody at 1:100 dilution.

  • Application

    Sample (30 µg of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Positive Control 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher. :

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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