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技術(shù)文章

MCM2 antibody Catalog Number：OM262648

點擊次數(shù)：27 發(fā)布時間：2024/9/27 14:44:14
 

Product Profile

Product Name MCM2 antibody
Antibody Type Primary Antibodies
Product description 
Mouse Monoclonal Antibody [2B3] to MCM2The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) ａnd may be involved in the formation of replication forks ａnd in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, ａnd 7, ａnd has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, ａnd thus regulated by, protein kinases CDC2 ａnd CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]
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Immunogen 
The immunogen corresponding to human MCM2 expressed in E. Coli.

Key Feature

Clonality Monoclonal
Isotype IgG1
Clone Number 2B3
Host Species Mouse
Tested Applications

ELISAFACSIHCIHC-PWB
 
ELISA: 1/10000.:
Species Reactivity

HumanMouseMonkeyRat
Concentration 1 mg/ml
Purification  

Target Information

Gene Synonyms
CDCL1
D3S3194
BM28
MITOTIN
CCNL1
CDC19
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Target MCM2
Molecular Weight(MW) 125 kDa (note)

Application

  • Application

    ELISA analysis of MCM2 antibody [2B3] using control (100 ng) ａnd antigen (10 ng, 50 ng, 100 ng).

  • Application

    WB analysis of PC-12 (1), Cos7 (2), NIH3T3 (3), HepG2 (4), HEK293 (5), K562 (6), Jurkat (7), HeLa (8) ａnd MCF-7 (9) cell lysates using MCM2 antibody [2B3].

  • Application

    FACS analysis of HeLa cells using MCM2 antibody [2B3] (blue) ａnd a negative control (red).

  • Application

    IHC-P analysis of colon cancer tissues using MCM2 antibody [2B3].

Application Notes
ELISA: 1/10000.:

Additional Information

Form Liquid
Storage Instructions 4°C; -20°C for long term storage
Storage Buffer Ascitic fluid containing 0.03% sodium azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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