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技術文章

MCM2 Catalog Number：OM640904

點擊次數(shù)：35 發(fā)布時間：2024/9/27 14:43:24
 

Product Profile

Product Name MCM2
Antibody Type Primary Antibodies
Immunogen 
Purified recombinant fragment of human MCM2 expressed in E. Coli.

Key Feature

Clonality Monoclonal
Host Species Unknow
Tested Applications

WBIHCICCFCMICC/IF
 
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
More 
Species Reactivity

Human

Target Information

Alternative Names
BM28
CCNL1
CDCL1
cdc19
D3S3194
MITOTIN
KIAA0030
MGC10606
More 
Molecular Weight(MW) 125kDa

Database Links

Entrez Gene 4171

Application

  • Application

    Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

  • Application

    Figure 1: Western blot analysis using MCM2 mAb against human MCM2 (AA: 16-232) recombinant protein. (Expected MW is 50.4 kDa)

  • Application

    Figure 2: Western blot analysis using MCM2 mouse mAb against MCF-7 (1), Hela (2), Jurkat (3), K562 (4), HEK293 (5) ａnd HEPG2 (6) cell lysate.

  • Application

    Figure 3: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MCM2 mouse mAb with DAB staining.

  • Application

    Figure 4: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MCM2 mouse mAb with DAB staining.

  • Application

    Figure 5: Immunofluorescence analysis of Hela cells using MCM2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Application

    Figure 6: Flow cytometric analysis of Jurkat cells using MCM2 mouse mAb (green) ａnd negative control (red).

Application Notes
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
More 

Additional Information

Form Ascitic fluid containing 0.03% sodium azide.
Storage Instructions 4℃; -20℃ for long term storage

原創(chuàng)作者：上海篤瑪生物科技有限公司

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