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MCM2 antibody , N-term Catalog Number：OM262649

點擊次數(shù)：27 發(fā)布時間：2024/9/27 14:38:37
 

Product Profile

Product Name MCM2 antibody , N-term
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to MCM2 (minichromosome maintenance complex component 2)The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) ａnd may be involved in the formation of replication forks ａnd in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, ａnd 7, ａnd has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, ａnd thus regulated by, protein kinases CDC2 ａnd CDC7. [provided by RefSeq]
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Immunogen 
Recombinant protein encompassing a sequence within the N-terminus region of human MCM2. The exact sequence is proprietary.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ICC/IFIPWB
 
*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity

Human
Predicted Reactivity Mouse, RatMouse (88%), Rat (88%)
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
BM28 antibody
CCNL1 antibody
CDCL1 antibody
D3S3194 antibody
KIAA0030 antibody
MGC10606 antibody
MITOTIN antibody
cdc19 antibody
MCM2 antibody
cell devision cycle-like 1 antibody
DNA replication licensing factor MCM2 antibody
nuclear protein BM2
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Molecular Weight(MW) 102 kDa (note)

Application

  • Application

    MCM2 antibody [N1N2], N-term immunoprecipitates MCM2 protein in IP experiments. IP samples: HCT-116 whole cell extract A. 40 μg HCT-116 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of MCM2 protein by 4 μg MCM2 antibody [N1N2], N-term 7.5 % SDS-PAGE The immunoprecipitated MCM2 protein was detected by MCM2 antibody [N1N2], N-term diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

  • Application

    MCM2 antibody [N1N2], N-term detects MCM2 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM2 protein stained by MCM2 antibody [N1N2], N-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

  • Application

    Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE diluted at 1:1000

Positive Control A549 , HeLa , HepG2 , HCT116
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher. :

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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