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技術(shù)文章

MCM2 Antibody Catalog Number：OM284350

點擊次數(shù)：27 發(fā)布時間：2024/9/27 14:31:02
 

Product Profile

Product Name MCM2 Antibody
Antibody Type Primary Antibodies
Product description 
The protein encoded by MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) ａnd may be involved in the formation of replication forks ａnd in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, ａnd 7, ａnd has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, ａnd thus regulated by, protein kinases CDC2 ａnd CDC7.1) Yu, Z., et al., (2004) J. Mol. Biol. 340 (5), 1197-1206.
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Immunogen 
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM2.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

ELISAWB
 
MCM2 antibody can be used for detection of MCM2 by ELISA at 1:312500. MCM2 antibody can be used for detection of MCM2 by western blot at 1.25 μg/mL, ａnd HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Species Reactivity

Human
Concentration 1 mg/ml
Purification Protein A

Target Information

Gene Symbol MCM2
Alternative Names
MCM2
BM28
CCNL1
CDCL1
cdc19
D3S3194
MITOTIN
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Molecular Weight(MW) 99 kDa

Database Links

Entrez Gene 4171
Protein Accession NP_004517

Application

  • Application

    Antibody used in WB on mouse neural stem at 1:1000.

  • Application

    Antibody used in WB on Human Jurkat 1.25 ug/ml.

Positive Control 1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
MCM2 antibody can be used for detection of MCM2 by ELISA at 1:312500. MCM2 antibody can be used for detection of MCM2 by western blot at 1.25 μg/mL, ａnd HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:

Additional Information

Form Liquid
Storage Instructions For short periods of storage (days) store at 4˚C. For longer periods of storage, store MCM2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt

原創(chuàng)作者：上海篤瑪生物科技有限公司

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