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技術(shù)文章

MCM2 Antibody Catalog Number：OM284353

點(diǎn)擊次數(shù)：37 發(fā)布時(shí)間：2024/9/26 14:48:49
 

Product Profile

Product Name MCM2 Antibody
Antibody Type Primary Antibodies
Product description 
MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) ａnd may be involved in the formation of replication forks ａnd in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, ａnd 7, ａnd has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, ａnd thus regulated by, protein kinases CDC2 ａnd CDC7.1) Saade, E., et al. Proteomics 9(21):4934-4943(2009)
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Immunogen 
This MCM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 649-677 amino acids from the C-terminal region of human MCM2.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

IHC-PWB
 
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:
Species Reactivity

HumanMouse
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol MCM2
Alternative Names
DNA replication licensing factor MCM2
Minichromosome maintenance protein 2 homolog
Nuclear protein BM28
MCM2
BM28
CCNL1
CDCL1
KIAA0030
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Molecular Weight(MW) 102 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Xenopus

Database Links

Entrez Gene 4171
Protein Accession P49736

Application

  • Application

    MCM2 Antibody immunohistochemistry analysis in formalin fixed ａnd paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody ａnd DAB staining.

  • Application

    Western blot analysis of MCM2 Antibody in HL-60 cell line lysates (35ug/lane)

  • Application

    Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lysates/proteins at 20 ug per lane.

Application Notes
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
 

原創(chuàng)作者：上海篤瑪生物科技有限公司

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