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技術(shù)文章

Prohibitin antibody Catalog Number：OM270566

點(diǎn)擊次數(shù)：31 發(fā)布時(shí)間：2024/9/26 14:45:18
 

Product Profile

Product Name Prohibitin antibody
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to Prohibitin (prohibitin)Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation ａnd may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3＇ untranslated region. The longer transcript is present at higher levels in proliferating tissues ａnd cells, suggesting that this longer 3＇ untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq]
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Immunogen 
Recombinant protein encompassing a sequence within the center region of human Prohibitin. The exact sequence is proprietary.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ICC/IFIHC-PWB
 
*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity

HumanMouseRat
Predicted Reactivity Zebrafish, Xenopus laevis, Dog, Cat, Chicken, Rhesus Monkey, Chimpanzee, BovineZebrafish (94%), Xenopus laevis (89%), Dog (100%), Cat (100%), Chicken (96%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%)
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Synonyms
PHB1 antibody
PHB antibody
prohibitin antibody
Molecular Weight(MW) 30 kDa (note) (5)
Cellular Localization Mitochondrion inner membrane

Application

  • Application

    Prohibitin antibody detects Prohibitin protein at mitochondria by immunofluorescent analysis. Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: Prohibitin protein stained by Prohibitin antibody diluted at 1:1000. Blue: Hoechst 33342 staining.

  • Application

    Prohibitin antibody detects PHB protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 12 % SDS-PAGE Prohibitin antibody dilution: 1:1000

  • Application

    Prohibitin antibody detects PHB protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract 12 % SDS-PAGE Prohibitin antibody dilution: 1:1000

  • Application

    Prohibitin antibody detects Prohibitin protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Prohibitin antibody dilution: 1:500.

  • Application

    Prohibitin antibody detects Prohibitin protein at mitochondria on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Prohibitin antibody dilution: 1:500.

  • Application

    Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Prohibitin antibody at 1:500 dilution.

  • Application

    Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 12% SDS PAGE diluted at 1:1000

Positive Control IMR32 , U87-MG , Neuro2A , GL261 , C8D30 , PC-12 , Rat2
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher. :

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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