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技術文章

Anti-PHB1 antibody Catalog Number:OM642067

點擊次數(shù):36 發(fā)布時間:2024/9/26 14:35:12
 

Product Profile

Product Name Anti-PHB1 antibody
Antibody Type Primary Antibodies
Immunogen
Recombinent Proteins

Key Feature

Clonality Monoclonal
Isotype IgG
Host Species Mouse
Tested Applications

ELISAICC/IFIHCWB

 
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Species Reactivity

HumanRabbit

Concentration 1 mg/ml
Purification Protein A

Target Information

Gene Symbol PHB
Gene Synonyms
PHB1
HEL-215
HEL-S-54e
Gene Full Name prohibitin
Gene Summary
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
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Molecular Weight(MW) 30 kDa
Source Mouse
Cellular Localization Cell membrane, Cylasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

Application

  • WB

    Western blot analysis using PHB1 mouse mAb against HT1080 (1), MCF-7 (2), NIH 3T3 (3), K562 (4) and COS-7 (5) cell lysate.

  • IHC

    Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using PHB1 antibody with DAB staining.

  • ICC/IF

    Immunofluorescence analysis of PC-3 cells using PHB1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500

Additional Information

Form Liquid
Storage Instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer Purified antibody in PBS with 0.05% sodium azide.

原創(chuàng)作者:上海篤瑪生物科技有限公司

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