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技術(shù)文章

Prohibitin Antibody Catalog Number：OM288898

點擊次數(shù)：58 發(fā)布時間：2024/9/26 14:19:12
 

Product Profile

Product Name Prohibitin Antibody
Antibody Type Primary Antibodies
Product description 
Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation ａnd may be a tumor suppressor. Mutations have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3＇ untranslated region.1) Gregory-Bass,R.C., Int. J. Cancer 122 (9), 1923-1930 (2008) Ross,J.A., J. Biol. Chem. 283 (8), 4699-4713 (2008) White,J.J., Genomics 11 (1), 228-230 (1991)
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Immunogen 
This Prohibitin (PHB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the N-terminal region of human Prohibitin (PHB1).

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

FACSIHC-PWB
 
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol PHB
Alternative Names
Prohibitin
PHB
Molecular Weight(MW) 30 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Bovine

Database Links

Entrez Gene 5245
Protein Accession P35232

Application

  • Application

    Western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PHB1 antibody detected the PHB1 protein (arrow).

  • Application

    Formalin-fixed ａnd paraffin-embedded human hepatocarcinoma with Prohibitin (PHB1) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Application

    Flow cytometric analysis of ATDC5 cells using PHB1 Antibody (N-term)(bottom histogram) compared to a negative control cell ( histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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