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技術(shù)文章

G6PD antibody Catalog Number：OM253796

點(diǎn)擊次數(shù)：40 發(fā)布時(shí)間：2024/9/25 15:21:21
 

Product Profile

Product Name G6PD antibody
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to G6PD (glucose-6-phosphate dehydrogenase)This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents ａnd in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity ａnd associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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Immunogen 
Recombinant protein encompassing a sequence within the center region of human G6PD. The exact sequence is proprietary.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

IHC-PWB
 
*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity

HumanMouseRat
Predicted Reactivity Xenopus laevis, Rabbit, Sheep, Rhesus Monkey, BovineXenopus laevis (82%), Rabbit (95%), Sheep (92%), Rhesus Monkey (99%), Bovine (95%)
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Synonyms
G6PD1 antibody
G6PD antibody
"glucose-6-phosphate dehydrogenase
G6PD antibody"
glucose-6-phosphate 1-dehydrogenase antibody
glucose-6-phosphate dehydrogenase antibody
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Molecular Weight(MW) 59 kDa (note) (2)

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded U87 xenograft, using G6PD antibody at 1:300 dilution.

  • Application

    Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, ａnd the membrane was blotted with G6PD antibody diluted at 1:500.

  • Application

    Sample (30 ug of whole cell lysate) A: HeLa S3 7.5% SDS PAGE diluted at 1:500

Positive Control 293T , A431 , H1299 , HeLaS3 , HepG2 , Raji , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher. :

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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