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技術(shù)文章

G6PD Catalog Number：OM638966

點(diǎn)擊次數(shù)：49 發(fā)布時(shí)間：2024/9/25 15:20:03
 

Product Profile

Product Name G6PD
Antibody Type Primary Antibodies
Product description 
Glucose-6-phosphate 1-dehydrogenase (G6PD) plays an important role in the pentose phosphate pathway. It is a member of the glucose-6-phosphate dehydrogenase family of proteins. G6PD is a ubiquitous enzyme that produces pentose sugars for nucleic acid synthesis, but is also involved in carbohydrate degradation, as it is one of the main producers of NADPH reducing power. G6PD has NADP as a co-factor ａnd structural element. It can be found as a homodimer or homotetramer, ａnd is primarily detected in lymphoblasts, granulocytes ａnd sperm. Defects in G6PD can cause chronic non-spherocytic hemolytic anemia (CNSHA), especially in areas in which malaria is an epidemic. Individuals with a high level of G6PD-deficiency are at higher risk of acute hemolytic attacks.
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Immunogen 
Recombinant protein.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHC
 
WB:1:500~1:2000
ICC:1:200~1:500
IHC:1:50~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
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Species Reactivity

HumanMouseRat
Concentration 1 mg/mL.

Target Information

Alternative Names
G6PD antibody G6PD_HUMAN antibody G6PD1 antibody G6pdx antibody Glucose 6 phosphate 1 dehydrogenase antibody Glucose 6 phosphate dehydrogenase antibody Glucose 6 phosphate dehydrogenase
G6PD antibody Glucose-6-phosphate 1-dehydrogenase antibody MET19 antibody POS10 antibody Zwf1p antibody
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Molecular Weight(MW) 59 kDa
Cellular Localization Cylasm. Nucleus. Membrane.

Database Links

SwissProt ID P11413
Q00612
P05370

Application

  • Application

    Fig1: Western blot analysis of G6PD on different lysates using anti-G6PD antibody at 1/1,000 dilution. Positive control：Lane1: A549Lane2: HelaLane3: MCF-7Lane4: PC-12Lane 5: Mouse spleen

  • Application

    Fig2: ICC staining G6PD in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining G6PD in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: ICC staining G6PD in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-G6PD antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-G6PD antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-G6PD antibody. Counter stained with hematoxylin.

  • Application

    Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-G6PD antibody. Counter stained with hematoxylin.

Positive Control A549, Hela, MCF-7, PC-12, mouse spleen tissue lysate, human tonsil tissue, human colon cancer tissue, human spleen tissue, mouse testis tissue.
Application Notes
WB:1:500~1:2000
ICC:1:200~1:500
IHC:1:50~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
More 

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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