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技術(shù)文章

G6PD Antibody Catalog Number：OM279344

點擊次數(shù)：39 發(fā)布時間：2024/9/25 15:16:44
 

Product Profile

Product Name G6PD Antibody
Antibody Type Primary Antibodies
Product description 
G6PD encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents ａnd in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity ａnd associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia.1) Louicharoen, C., et al. Science 326(5959):1546-1549(2009)
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Immunogen 
This G6PD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-326 amino acids from the Central region of human G6PD.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

FACSIHC-PWB
 
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml

Target Information

Gene Symbol G6PD
Alternative Names
Glucose-6-phosphate 1-dehydrogenase
G6PD
G6PD
Molecular Weight(MW) 59 kDa

Database Links

Entrez Gene 2539
Protein Accession P11413

Application

  • Application

    Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell ( histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Application

    Western blot analysis of G6PD Antibody in K562,MCF-7 cell line lysates (35ug/lane)

  • Application

    G6PD Antibody IHC analysis in formalin fixed ａnd paraffin embedded testis followed by peroxidase conjugation of the secondary antibody ａnd DAB staining.

Application Notes
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
 

原創(chuàng)作者：上海篤瑪生物科技有限公司

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