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技術文章

NQO1 Antibody (Center) Catalog Number：OM215597

點擊次數(shù)：33 發(fā)布時間：2024/9/25 14:14:18
 

Product Profile

Product Name NQO1 Antibody (Center)
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WBFCIF
 
WB~~1:50~100 FC~~1:10~50 IF~~1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml
Purification  

Target Information

Gene Synonyms
DIA4
NMOR1
Alternative Names
NQO1
DIA4
NMOR1
NAD(P)H dehydrogenase [qui] 1
Azoreductase
DT-diaphorase
Menadione reductase
NAD(P)H:qui oxidoreductase 1
Phylloqui reductase
Qui reductase 1
More 
Molecular Weight(MW) 30868 Da
Function
The enzyme apparently serves as a qui reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis
Tissue Specificity This NQO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124~153 amino acids from the Center region of human NQO1.
Cellular Localization Cylasm.

Database Links

Entrez Gene 1728

Application

  • Application

    Western blot analysis of NQO1 antibody (Center) in NCI-H460 cell line lysates (35ug/lane). NQO1 (arrow) was detected using the purified Pab.

  • Application

    NQO1 antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell ( histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Application

    Confocal immunofluorescent analysis of NQO1 Antibody (Center)(Cat#AP7350c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Application Notes
WB~~1:50~100 FC~~1:10~50 IF~~1:10~50:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
 

原創(chuàng)作者：上海篤瑪生物科技有限公司

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