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NQO1 antibody Catalog Number：OM265892

點擊次數(shù)：23 發(fā)布時間：2024/9/25 14:11:10
 

Product Profile

Product Name NQO1 antibody
Antibody Type Primary Antibodies
Product description 
Mouse monoclonal antibody to NQO1NQO1 is a 2-electron reductase that detoxifies quis derived from the oxidation of phenolic metabolites of benzene. Individuals homozygous for the T/T form of the 609C-T polymorphism have an increased risk of benzene hematotoxicity.
Immunogen 
Full length recombinant NQO1 (from human lung).

Key Feature

Clonality Monoclonal
Isotype IgG1
Clone Number A180
Host Species Mouse
Tested Applications

ICC/IFIHC-FIHC-PIPWB
 
This antibody works well in Western blot where a single band at ~31 kDa is seen. No other applications have been tested. Past productions of this antibody have been used for immunoprecipitation, immunofluorescence, ａnd immunohistochemistry (in human, monkey, ａnd canine formalin-fixed paraffin-embedded sections, frozen sections not tested).Suggested working dilutions: *Western blot: 1:1,000-1:10,000 (ECL)Immunohistochemistry: (+)Immunofluorescence: (+) Immunoprecipitation: (+)*The investigator should determine the optimal working dilution for a specific application.:
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Species Reactivity

HumanMouseDogMonkeyRat
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)

Target Information

Gene Synonyms
DHQU
DIA4
DTD
NMOR1
NMORI
QR1
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Target NQO1
Tissue Specificity This antibody is specific for NQO1. It does not cross-react with NQO2.
Cellular Localization Cylasmic (primarily), nuclear (faintly)

Application

  • Application

    Immunocytochemistry/Immunofluorescence: NQO1 Antibody (A180) [ - NQO1 Antibody (A180) [ - NQO1 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei ａnd beta-tubulin were counterstained with DAPI (blue) ａnd Dylight 550 (red).

  • Application

    Immunohistochemistry: NQO1 Antibody (A180) [ - Analysis of NQO1 in human breast cancer using DAB with hematoxylin counterstain.

  • Application

    Western Blot: NQO1 Antibody (A180) [ - Detection of NQO1 in A549 lysates using Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.

  • Application

     

Application Notes
This antibody works well in Western blot where a single band at ~31 kDa is seen. No other applications have been tested. Past productions of this antibody have been used for immunoprecipitation, immunofluorescence, ａnd immunohistochemistry (in human, monkey, ａnd canine formalin-fixed paraffin-embedded sections, frozen sections not tested).Suggested working dilutions: *Western blot: 1:1,000-1:10,000 (ECL)Immunohistochemistry: (+)Immunofluorescence: (+) Immunoprecipitation: (+)*The investigator should determine the optimal working dilution for a specific application.:
More 

Additional Information

Form Liquid
Storage Instructions Aliquot into working amounts ａnd store at -20C. Avoid freeze/thaw cylces.
Storage Buffer Tris-glycine ａnd 150mM NaCl containing 0.05% sodium azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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