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技術(shù)文章

NQO1 [13A1] Catalog Number：OM628315

點(diǎn)擊次數(shù)：64 發(fā)布時(shí)間：2024/9/24 15:04:16
 

Product Profile

Product Name NQO1 [13A1]
Antibody Type Primary Antibodies
Product description 
NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals ａnd radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression ａnd coordinated induction of NQO1. ARE activation by tert-butylhydroqui is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B ａnd Jun-D bind to the ARE ａnd regulate expression ａnd induction of NQO1 gene. Maf-Maf homodimers ａnd possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE ａnd do not repress NQO1 transcription.
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Immunogen 
Recombinant protein within Human NQO1 aa 1-285.

Key Feature

Clonality Monoclonal
Isotype IgG2b
Host Species Mouse
Tested Applications

WBIHC
 
WB:1:1,000-1:5,000:
IHC:1:50-1:100:
Species Reactivity

HumanRat
Concentration 2 mg/mL.

Target Information

Alternative Names
Azoreductase antibody Cytochrome b 5 reductase antibody DHQU antibody DIA 4 antibody DIA4 antibody Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody Diaphorase (NADH/NADPH) antibody Diaphorase 4 antibody Dioxin inducible 1 antibody DT diaphorase antibody DT-diaphorase antibody DTD antibody Menadione reductase antibody NAD(P)H dehydrogenase [qui] 1 antibody NAD(P)H dehydrogenase qui 1 antibody NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody NAD(P)H qui dehydrogenase 1 antibody NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody NAD(P)H:menadione oxidoreductase 1 antibody NAD(P)H:Qui acceptor oxidoreductase type 1 antibody NAD(P)H:qui oxidoreductase 1 antibody NAD(P)H:qui oxireductase antibody NMOR 1 antibody NMOR I antibody NMOR1 antibody NMORI antibody NQO 1 antibody NQO1 antibody NQO1_HUMAN antibody Phylloqui reductase antibody QR 1 antibody QR1 antibody Qui reductase 1 antibody
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Molecular Weight(MW) 31 kDa
Cellular Localization Cylasm.

Database Links

SwissProt ID
P15559
P05982

Application

  • Application

    Fig1: Western blot analysis of NQO1 on different lysates. Proteins were transferred to a PVDF membrane ａnd blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysate, untreated Lane 2: A549 cell lysate, untreated

  • Application

    Fig2: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-NQO1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O ａnd PBS, ａnd then probed with the antibody (EM1801-21) at 1/50 dilution, for 30 minutes at room temperature ａnd detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin ａnd mounted with DPX.

  • Application

    Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NQO1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O ａnd PBS, ａnd then probed with the antibody (EM1801-21) at 1/50 dilution, for 30 minutes at room temperature ａnd detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin ａnd mounted with DPX.

Positive Control SiHa, A549, rat uterus tissue, human kidney tissue.
Application Notes
WB:1:1,000-1:5,000:
IHC:1:50-1:100:

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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