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NQO1 Catalog Number：OM628927

點(diǎn)擊次數(shù)：44 發(fā)布時間：2024/9/24 15:03:32
 

Product Profile

Product Name NQO1
Antibody Type Primary Antibodies
Product description 
NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals ａnd radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression ａnd coordinated induction of NQO1. ARE activation by tert-butylhydroqui is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B ａnd Jun-D bind to the ARE ａnd regulate expression ａnd induction of NQO1 gene. Maf-Maf homodimers ａnd possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE ａnd do not repress NQO1 transcription.
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Immunogen 
Recombinant protein within human NQO1 1-250 aa.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBIHCFC
 
WB:1:500-1:1,000
IHC:1:50-1:200
FC:1:50-1:100
Species Reactivity

HumanMouseRat
Concentration 1 mg/mL.

Target Information

Alternative Names
Azoreductase antibody Cytochrome b 5 reductase antibody DHQU antibody DIA 4 antibody DIA4 antibody Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody Diaphorase (NADH/NADPH) antibody Diaphorase 4 antibody Dioxin inducible 1 antibody DT diaphorase antibody DT-diaphorase antibody DTD antibody Menadione reductase antibody NAD(P)H dehydrogenase [qui] 1 antibody NAD(P)H dehydrogenase qui 1 antibody NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody NAD(P)H qui dehydrogenase 1 antibody NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody NAD(P)H:menadione oxidoreductase 1 antibody NAD(P)H:Qui acceptor oxidoreductase type 1 antibody NAD(P)H:qui oxidoreductase 1 antibody NAD(P)H:qui oxireductase antibody NMOR 1 antibody NMOR I antibody NMOR1 antibody NMORI antibody NQO 1 antibody NQO1 antibody NQO1_HUMAN antibody Phylloqui reductase antibody QR 1 antibody QR1 antibody Qui reductase 1 antibody
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Molecular Weight(MW) 31 kDa
Cellular Localization Cylasm.

Database Links

SwissProt ID P15559
Q64669
P05982

Application

  • Application

    Fig1: Western blot analysis of NQO1 on different cell lysates using anti-NQO1 antibody at 1/1,000 dilution. Positive control： Lane1: Siha Lane2: A549

  • Application

    Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-NQO1 antibody. Counter stained with hematoxylin.

  • Application

    Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-NQO1 antibody. Counter stained with hematoxylin.

  • Application

    Fig4: Flow cytometric analysis of Siha cells with NQO1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Positive Control Siha, A549, rat kidney tissue, mouse colon tissue.
Application Notes
WB:1:500-1:1,000
IHC:1:50-1:200
FC:1:50-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide

原創(chuàng)作者：上海篤瑪生物科技有限公司

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