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技術(shù)文章

NQO1 Antibody Catalog Number:OM286504

點(diǎn)擊次數(shù):37 發(fā)布時(shí)間:2024/9/24 14:53:57
 

Product Profile

Product Name NQO1 Antibody
Antibody Type Primary Antibodies
Product description
NQO1 is a member of the NAD(P)H dehydrogenase(qui) family and a cylasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quis to hydroquis. This protein's enzymatic activity prevents the one electron reduction of quis that results in the production of radical species. Altered expression of the protein has been seen in many tumors and is also associated with Alzheimer's disease (AD).1) Hubackova,M., Vaclavikova,R. Pharmacogenet. Genomics 19 (7), 505-512 (2009) Canova,C., Hashibe,M. Cancer Res. 69 (7), 2956-2965 (2009) Li,Y. and Jaiswal,A.K. J. Biol. Chem. 267 (21), 15097-15104 (1992)
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Immunogen
This NQO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-144 amino acids from the Central region of human NQO1.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

FACSIFWB

 
For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:
Species Reactivity

Human

Concentration 1 mg/ml

Target Information

Gene Symbol NQO1
Alternative Names
NAD(P)H dehydrogenase [qui] 1
Azoreductase
DT-diaphorase
DTD
Menadione reductase
NAD(P)H:qui oxidoreductase 1
Phylloqui reductase
Qui reductase 1
QR1
NQO1
DIA4
NMOR1
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Molecular Weight(MW) 31 kDa

Database Links

Entrez Gene 1728
Protein Accession P15559

Application

  • Application

    Confocal immunofluorescent analysis of NQO1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Application

    Western blot analysis of NQO1 antibody in NCI-H460 cell line lysates (35ug/lane)

  • Application

    Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell ( histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes
For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.

原創(chuàng)作者:上海篤瑪生物科技有限公司

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