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技術(shù)文章

PPP1CA Antibody Catalog Number：OM288645

點(diǎn)擊次數(shù)：26 發(fā)布時(shí)間：2024/9/24 14:20:26
 

Product Profile

Product Name PPP1CA Antibody
Antibody Type Primary Antibodies
Product description 
PPP1CA is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, ａnd HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human ａnd mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning ａnd memory.The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, ａnd HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human ａnd mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning ａnd memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.1) Lesage, B., (2007) Biochemistry 46 (31), 8909-8919.
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Immunogen 
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PPP1CA.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

ELISAWB
 
PPP1CA antibody can be used for detection of PPP1CA by ELISA at 1:1562500. PPP1CA antibody can be used for detection of PPP1CA by western blot at 1 μg/mL, ａnd HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Species Reactivity

DogHumanMouseRat
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol PPP1CA
Alternative Names
PPP1CA
MGC15877
MGC1674
PP-1A
PPP1A
PP1A
PP1alpha
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Molecular Weight(MW) 38 kDa

Database Links

Entrez Gene 5499
Protein Accession NP_001008709

Application

  • Application

    Antibody used in WB on HEK293, H1299 at 1:1000 (Lane 1: 40ug HEK293 lysate Lane 2: 40ug H1299 lysate ).

  • Application

    Antibody used in WB on Human Jurkat 0.2-1 ug/ml.

Positive Control 1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
PPP1CA antibody can be used for detection of PPP1CA by ELISA at 1:1562500. PPP1CA antibody can be used for detection of PPP1CA by western blot at 1 μg/mL, ａnd HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:

Additional Information

Form Liquid
Storage Instructions For short periods of storage (days) store at 4˚C. For longer periods of storage, store PPP1CA antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
 

原創(chuàng)作者：上海篤瑪生物科技有限公司

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