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技術(shù)文章

PPP2R4 antibody Catalog Number：OM269455

點(diǎn)擊次數(shù)：27 發(fā)布時(shí)間：2024/9/24 14:12:40
 

Product Profile

Product Name PPP2R4 antibody
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to PPP2R4 (protein phosphatase 2A activator, regulatory subunit 4)Protein phosphatase 2A is one of the four major Ser/Thr phosphatases ａnd is implicated in the negative control of cell growth ａnd division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, ａnd a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B＇/PR61, ａnd B＇＇/PR72 families. These different regulatory subunits confer distinct enzymatic specificities ａnd intracellular localizations to the holozenzyme. The product of this gene belongs to the B＇ family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
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Immunogen 
Recombinant protein encompassing a sequence within the center region of human PPP2R4. The exact sequence is proprietary.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ICC/IFWB
 
*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity

Human
Predicted Reactivity Mouse, Rat, RabbitMouse (96%), Rat (95%), Rabbit (94%)
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Synonyms
MGC2184 antibody
PP2A antibody
PR53 antibody
PTPA antibody
PPP2R4 antibody
PP2A subunit B＇ isoform PR53 antibody
phosphotyrosyl phosphatase activator antibody
serine/threonine-protein phosphatase 2A activator antibody
PP2A phosphatase activator ant
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Molecular Weight(MW) 41 kDa (note) (3)

Application

  • Application

    Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PPP2R4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.

  • Application

    Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:1000

Positive Control A549 , HeLa , HepG2 , HCT116
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher. :

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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