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技術(shù)文章

PPP2R4 antibody Catalog Number：OM269460

點擊次數(shù)：24 發(fā)布時間：2024/9/24 14:03:41
 

Product Profile

Product Name PPP2R4 antibody
Antibody Type Primary Antibodies
Product description 
Mouse Monoclonal Antibody [4D9] to PPP2R4Protein phosphatase 2A is one of the four major Ser/Thr phosphatases ａnd is implicated in the negative control of cell growth ａnd division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, ａnd a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B＇/PR61, ａnd B＇＇/PR72 families. These different regulatory subunits confer distinct enzymatic specificities ａnd intracellular localizations to the holozenzyme. The product of this gene belongs to the B＇ family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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Immunogen 
The immunogen corresponding to human PPP2R4 expressed in E. Coli.

Key Feature

Clonality Monoclonal
Isotype IgG1
Clone Number 4D9
Host Species Mouse
Tested Applications

ELISAFACSICC/IFIHCIHC-PWB
 
ELISA: 1/10000.:
Species Reactivity

Human
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification  

Target Information

Gene Synonyms
PR53
PP2A
PTPA
Target PPP2R4
Molecular Weight(MW) 41 kDa (note)

Application

  • Application

    ELISA analysis of control antigen ａnd antigen using PPP2R4 antibody [4D9].

  • Application

    FACS analysis of MCF-7 cells using PPP2R4 antibody [4D9] (green) or a negative control (red).

  • Application

    ICC/IF analysis of HeLa cells using PPP2R4 antibody [4D9] (green), Alexa Fluor-555 phalloidin (red) ａnd DRAQ5 fluorescent DNA dye (blue).

  • Application

    IHC-P analysis of cervical cancer tissues using PPP2R4 antibody [4D9].

  • Application

    IHC-P analysis of lung cancer tissues using PPP2R4 antibody [4D9].

  • Application

    WB analysis of human PPP2R4 (a.a. 1-154) recombinant protein using PPP2R4 antibody [4D9].

Application Notes
ELISA: 1/10000.:

Additional Information

Form Liquid
Storage Instructions 4°C; -20°C for long term storage
Storage Buffer Purified antibody in PBS with 0.05% sodium azide ａnd 0.5% protein stabilizer.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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