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Product Name	alpha Tubulin antibody (ascites)
Antibody Type	Primary Antibodies
Product description	Mouse monoclonal antibody [DM1A] to alpha TubulinTubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural ａnd mobile elements in mitosis, intracellular transport, flagellar movement ａnd in the cytoskeleton. Tubulin is a heterodimer which consists of alpha-tubulin ａnd beta-tubulin; both subunits have a molecular weight of approximately 50 kDa ａnd share considerable homology. The most widely studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.GT211302 may be used to study the intracellular organization ａnd distribution of tubulin along the static ａnd dynamic aspects of the cytoskeleton. The product can be used for the immunocytochemical localization of the alpha chain of tubulin by means of indirect immunofluorescent labeling of cultured cells, formalin fixed or frozen tissues or for specific identification of the alpha-tubulin band in immunoblotting or immunoprecipitation techniques. More
Immunogen	Purified chick brain tubulin.

Clonality	Monoclonal
Isotype	IgG1
Clone Number	DM1A
Host Species	Mouse
Tested Applications	ICC/IFIHC-PIPWBIB
	IF: Use at a dilution of 1/500, this is determined by indirect immunofluorescent labeling of cultured chicken fibroblasts. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 50 kDa. Optimal dilutions/concentrations should be determined by the end user.: More
Species Reactivity	HumanMouseAmphibiansFishFungiHamsterBovine
Concentration	1 mg/ml
Purification	Affinity purified

Gene Synonyms	TUBA1 P68366 alpha Tubulin 191110 H2ALPHA H2-ALPHA 7277 FLJ30169 TUBA1B TUBA2 TUBA3D TUBA8 H2 ALPHA More
Target	alpha Tubulin
Cellular Localization	Major constituent of microtubules.

Application

Chinese hamster embryonic fibroblasts in anaphase of mitosis were stained using Monoclonal Anti-α-Tubulin, clone no. DM1A with Anti-Mouse-Alexa 488 (green) ａnd DAPI (blue).
Application

Confocal image of the mouth of the Cryptocaryon irritans, a parasite of marine fishes, (theront stage) stained with Monoclonal Anti-α-Tubulin, clone no. DM 1A ａnd Anti-Mouse IgG-lissamine-rhodamine conjugate. Note the large bundles of microtubules which form a well-developed cyhyrigeal basket.
Application

IHC-P analysis of human heart sections using alpha Tubulin antibody [DM1A] at a dilution of 1:500.
Application

WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK ａnd (9) MDCK lysates using alpha Tubulin antibody [DM1A] at a dilution of 1:500.
Application

Whole cell extract of chicken gizzard was separated on SDS-PAGE ａnd blotted with Monoclonal Anti-α-Tubulin . The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate ａnd AEC substrate. Lane A: Antibody dilution 1:1,000 Lane B: Negative control (only secondary antibody)
Application

Human skin fibroblasts stained with Monoclonal Anti-α-Tubulin, clone No. DM1A .

Positive Control	Chicken fibroblasts
Application Notes	IF: Use at a dilution of 1/500, this is determined by indirect immunofluorescent labeling of cultured chicken fibroblasts. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 50 kDa. Optimal dilutions/concentrations should be determined by the end user.: More

Form	Liquid
Storage Instructions	Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot ａnd store at -20ºC or below. Avoid freeze-thaw cycles.
Storage Buffer	Raw Ascites containing 15mM sodium azide

原創(chuàng)作者：上海篤瑪生物科技有限公司

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