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技術(shù)文章

Bmi1 [B3-G5] Catalog Number：OM628508

點(diǎn)擊次數(shù)：43 發(fā)布時(shí)間：2024/9/20 15:48:20
 

Product Profile

Product Name Bmi1 [B3-G5]
Antibody Type Primary Antibodies
Product description 
The Bmi-1 was identified initially as an oncogene that cooperates with c-myc in the generation of B-cell lymphoma. It contributes to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, ａnd oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, ａnd cell-cycle arrest.
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Immunogen 
Recombinant protein within human Bmi1 full sequence.

Key Feature

Clonality Monoclonal
Isotype IgG2a
Host Species Mouse
Tested Applications

WBICCIHCFC
 
WB:1:1,000
IHC:1:200
ICC:1:200
FC:1:100-1:200
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Species Reactivity

HumanMouseRat
Concentration 2 mg/mL.

Target Information

Alternative Names
B lymphoma Mo MLV insertion region (mouse) antibody B lymphoma Mo MLV insertion region 1 homolog antibody Bmi 1 antibody BMI1 antibody BMI1 polycomb ring finger oncogene antibody BMI1_HUMAN antibody Flvi 2/bmi 1 antibody FLVI2/BMI1 antibody MGC12685 antibody Murine leukemia viral (bmi 1) oncogene homolog antibody Oncogene BMI 1 antibody PCGF 4 antibody PCGF4 antibody Polycomb complex protein BMI 1 antibody Polycomb complex protein BMI-1 antibody Polycomb group protein Bmi1 antibody Polycomb group ring finger 4 antibody Polycomb group RING finger protein 4 antibody RING finger protein 51 antibody RNF 51 antibody RNF51 antibody
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Molecular Weight(MW) 37kDa
Cellular Localization Nucleus, Cylasm

Database Links

SwissProt ID P35226
P25916

Application

  • Application

    Fig1: Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1,000 dilution.Positive control:Lane 1: 293T             Lane 2: JurkatLane 3: Hela              Lane 4: MCF-7Lane 5: HepG2           Lane 6: NIH/3T3Lane 7: PC12             Lane 8: Mouse kidneyLane 9: Human kidney     Lane 10: K562Lane 11: Human brain

  • Application

    Fig2: ICC staining Bmi1 in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining Bmi1 in Lovo cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: ICC staining Bmi1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMI1 antibody. Counter stained with hematoxylin.

  • Application

    Fig8: Flow cytometric analysis of Hela cells with BMI1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

Positive Control Hela, HepG2, Jurkat, 293T, MCF-7, PC12, K562, NIH/3T3, A549, LOVO, human tonsil tissue, human colon cancer tissue, human breast cancer tissue, mouse kidney tissue, human kidney tissue, human brain tissue.
Application Notes
WB:1:1,000
IHC:1:200
ICC:1:200
FC:1:100-1:200
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Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
 

原創(chuàng)作者：上海篤瑪生物科技有限公司

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