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聯(lián)系人:楊燕燕

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技術(shù)文章

BMI1 Catalog Number:OM640906

點擊次數(shù):44 發(fā)布時間:2024/9/20 15:45:53
 
Antibody Type Primary Antibodies
Immunogen
Purified recombinant fragment of human BMI1 expressed in E. Coli.

Key Feature

Clonality Monoclonal
Host Species Unknow
Tested Applications

WBIHCICCFCMICC/IF

 
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
More 
Species Reactivity

Human

Target Information

Alternative Names
PCGF4
RNF51
MGC12685
FLVI2/BMI1
More 
Molecular Weight(MW) 37kDa

Database Links

Entrez Gene 648

Application

  • Application

    Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

  • Application

    Figure 1: Western blot analysis using BMI1 mAb against human BMI1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)

  • Application

    Figure 2: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using BMI1 mouse mAb with DAB staining.

  • Application

    Figure 2: Western blot analysis using BMI1 mAb against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.

  • Application

    Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using BMI1 mouse mAb with DAB staining.

  • Application

    Figure 4: Immunofluorescence analysis of Hela cells using BMI1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Application

    Figure 5: Flow cytometric analysis of NIH/3T3 cells using BMI1 mouse mAb (green) and negative control (red).

Application Notes
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
More 

Additional Information

Form Ascitic fluid containing 0.03% sodium azide.
Storage Instructions 4℃; -20℃ for long term storage

原創(chuàng)作者:上海篤瑪生物科技有限公司

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