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技術(shù)文章

Bmi1 antibody Catalog Number：OM241672

點擊次數(shù)：90 發(fā)布時間：2024/9/20 15:43:52
 

Product Profile

Product Name Bmi1 antibody
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to Bmi1 (BMI1 polycomb ring finger oncogene)Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling ａnd modification of histones; it mediates monoubiquitination of histone H2A ＇Lys-119＇, rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
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Immunogen 
Recombinant protein encompassing a sequence within the center region of human Bmi1. The exact sequence is proprietary.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

IPWB
 
*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity

Human
Predicted Reactivity Mouse, Rat, Xenopus laevis, Dog, Cat, Pig, Chicken, Chimpanzee, BovineMouse (96%), Rat (92%), Xenopus laevis (82%), Dog (98%), Cat (99%), Pig (99%), Chicken (94%), Chimpanzee (100%), Bovine (98%)
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
FLVI2/BMI1
PCGF4
BMI1
BMI1 polycomb ring finger oncogene
RNF51
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Target Bmi1
Molecular Weight(MW) 37 kDa (note)
Cellular Localization Nucleus , Cylasm

Application

  • Application

    Bmi1 antibody detects Bmi1 protein by western blot analysis. A. 30 μg K562 whole cell lysate/extract B. 30 μg THP-1 whole cell lysate/extract 10 % SDS-PAGE Bmi1 antibody dilution: 1:3000

  • Application

    Bmi1 antibody immunoprecipitates Bmi1 protein in IP experiments. IP samples: K562 whole cell extract A. 40 μg K562 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of Bmi1 protein by 4 μg Bmi1 antibody 5 % SDS-PAGE The immunoprecipitated Bmi1 protein was detected by Bmi1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

Positive Control K562 , THP-1
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher. :

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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