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TNNI2 [A8-F12] Catalog Number：OM627677

點(diǎn)擊次數(shù)：27 發(fā)布時(shí)間：2024/9/20 15:36:13
 

Product Profile

Product Name TNNI2 [A8-F12]
Antibody Type Primary Antibodies
Product description 
Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable ａnd labile structures ａnd are crucial components of microvilli ａnd the contractile apparatus of muscle cells. Myosin is a hexamer of 2 heavy chains (MHC) ａnd 4 light chains (MLC) that interacts with actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis ａnd muscle contraction. Troponin facilitates the interaction between actin ａnd myosin by binding to calcium. Troponin is made up of at least two subunits, which are skeletal muscle skeletal muscle ａnd slow skeletal muscle. Structures of skeletal muscle troponin are composed of Troponin C (the sensor), Troponin I (the regulator), ａnd Troponin T (the link to the the regulator), ａnd Troponin T C is dumbbell-shaped ａnd has a hydrophobic pocket that increases the contractile force of muscle fibers. Troponin C has 2 isoforms: fast ａnd slow. Fast troponin C has two calci um binding sites while slow / cardiac troponin C has a single calcium binding site.
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Immunogen 
Recombinant protein

Key Feature

Clonality Monoclonal
Isotype IgG1
Host Species Mouse
Tested Applications

WBIHCFC
 
WB:1:500-1:1,000
IHC:1:100-1:200
FC:1:100-1:200
Species Reactivity

Human
Concentration 2 mg/mL.

Target Information

Alternative Names
AMCD 2B antibody AMCD2B antibody DA 2B antibody DA2B antibody fast skeletal muscle antibody Fast twitch skeletal muscle troponin I antibody fast-twitch isoform antibody FSSV antibody fsTnI antibody TNNI 2 antibody Tnni2 antibody TNNI2_HUMAN antibody tro antibody Troponin I antibody Troponin I fast skeletal muscle antibody Troponin I fast twitch 2 antibody Troponin I fast twitch isoform antibody Troponin I fast twitch skeletal muscle isoform antibody Troponin I skeletal fast antibody Troponin I type 2 (skeletal fast) antibody Troponin I type 2 antibody
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Molecular Weight(MW) 21 kDa
Cellular Localization Nucleus. Cytosol.

Database Links

SwissProt ID P48788

Application

  • Application

    Fig1: Western blot analysis of TNNI2 on human TNNI2 recombinant protein using anti-TNNI2 antibody at 1/1,000 dilution.

  • Application

    Fig2: Western blot analysis of TNNI2 on HEK293 (1) ａnd TNNI2-hIgGFc transfected HEK293 (2) cell lysate using anti-TNNI2 antibody at 1/1,000 dilution.

  • Application

    Fig3: Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue using anti-TNNI2 antibody. Counter stained with hematoxylin.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded human striated muscle tissue using anti-TNNI2 antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Flow cytometric analysis of NIH/3T3 cells with TNNI2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).

Positive Control Human TNNI2 recombinant protein, TNNI2-hIgGFc transfected HEK293 cell lysate, human cardiac muscle tissue, human striated muscle tissue, NIH-3T3.
Application Notes
WB:1:500-1:1,000
IHC:1:100-1:200
FC:1:100-1:200

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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