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技術(shù)文章

NF-L antibody , C-term Catalog Number：OM265300

點擊次數(shù)：21 發(fā)布時間：2024/9/20 15:19:01
 

Product Profile

Product Name NF-L antibody , C-term
Antibody Type Primary Antibodies
Product description 
Rabbit Monoclonal antibody [EP675Y] to NF-LNeuofilaments (NF) are members of the intermediate filament protein family also known as 10nm filaments. These neuron-specific filaments are the major constituents of the axonal cytoskeleton . NFs are composed of three major proteins; NF-H, NF-L ａnd NF-M ａnd the ratio of each protein changes during neuronal development. NF-H is the heavy molecular weight protein (200 kDa), NF-M the medium (160 kDa) ａnd NF-L the light protein (68kDa) . NF-L self polymerizes ａnd provides a scaffold for NFs to form via cross-bridging with NF-H or NF-M . NF-L aggregation has been linked to the pathogenesis of neurodegenerative disease, such as degradation of motor neurons .
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Immunogen 
A synthetic peptide corresponding to residues near the C-term of human NF-L was used as immunogen.

Key Feature

Clonality Monoclonal
Isotype IgG
Clone Number EP675Y
Host Species Rabbit
Tested Applications

FACSIPWB
 
For WB: Use at a dilution of 1:1,000:
Species Reactivity

HumanMouseRat
Concentration 1 mg/ml

Target Information

Gene Synonyms
NF68
NEFL
CMT2E
NFL
CMT1F
NF-L
FLJ53642
NF L
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Target NF-L
Molecular Weight(MW) 68 (note)

Application

  • Application

    A. Western blot analysis on SH-SY5Y lysate using anti-NF-L RabMAb (cat. # ) dilution 1:50000

Application Notes
For WB: Use at a dilution of 1:1,000:

Additional Information

Form Liquid
Storage Instructions Store at -20 °C. Stable for 12 months from date of receipt.
Storage Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide ａnd 0.05% BSA.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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