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技術(shù)文章

NEFL Catalog Number:OM640884

點(diǎn)擊次數(shù):110 發(fā)布時間:2024/9/19 14:30:37
 

Product Profile

Product Name NEFL
Antibody Type Primary Antibodies
Immunogen
Purified recombinant fragment of human NEFL expressed in E. Coli.

Key Feature

Clonality Monoclonal
Host Species Unknow
Tested Applications

WBIHCICCFCMICC/IF

 
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
More 
Species Reactivity

Human

Target Information

Alternative Names
NFL
NF-L
NF68
CMT1F
CMT2E
More 
Molecular Weight(MW) 62kDa

Database Links

Entrez Gene 4747

Application

  • Application

    Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

  • Application

    Figure 1: Western blot analysis using NEFL mAb against human NEFL (AA: 422-543) recombinant protein. (Expected MW is 62 kDa)

  • Application

    Figure 2: Western blot analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.

  • Application

    Figure 3: Immunofluorescence analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Application

    Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NEFL mouse mAb with DAB staining.

  • Application

    Figure 4: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using NEFL mouse mAb with DAB staining.

  • Application

    Figure 4: Flow cytometric analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).

Application Notes
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
More 

Additional Information

Form Ascitic fluid containing 0.03% sodium azide
Storage Instructions 4℃; -20℃ for long term storage
 

原創(chuàng)作者:上海篤瑪生物科技有限公司

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