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技術(shù)文章

NEFL Antibody (Center R185)

點(diǎn)擊次數(shù):52 發(fā)布時(shí)間:2024/9/19 14:29:06
 

Product Profile

Product Name NEFL Antibody (Center R185)
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WBIHCIF

 
WB~~1:50~100 IHC~~1:50~100 IF~~1:10~50:
Species Reactivity

Human

Concentration 1 mg/ml
Purification  

Target Information

Gene Synonyms
NF68
NFL
Alternative Names
NEFL
NF68
NFL
Neurofilament light polypeptide
68 kDa neurofilament protein
Neurofilament triplet L protein
More 
Molecular Weight(MW) 61517 Da
Function
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber
Tissue Specificity This NEFL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177~207 amino acids from the Center region of human NEFL.

Database Links

Entrez Gene 4747

Application

  • Application

    Western blot analysis of NEFL antibody (Center R185) in Y79 cell line lysates (35ug/lane). NEFL (arrow) was detected using the purified Pab.

  • Application

    NEFL Antibody (Center R185) (RB18878) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NEFL Antibody (Center R185) for immunohistochemistry. Clinical relevance has not been evaluated.

  • Application

    Confocal immunofluorescent analysis of NEFL Antibody (Center R185) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Application Notes
WB~~1:50~100 IHC~~1:50~100 IF~~1:10~50:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
 

原創(chuàng)作者:上海篤瑪生物科技有限公司

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