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技術文章

Fibrinogen gamma antibody Catalog Number：OM253131

點擊次數(shù)：64 發(fā)布時間：2024/9/19 14:26:18
 

Product Profile

Product Name Fibrinogen gamma antibody
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to Fibrinogen gamma (fibrinogen gamma chain)The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen ａnd fibrin regulate cell adhesion ａnd spreading, display vasoconstrictor ａnd chemotactic activities, ａnd are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia ａnd thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]
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Immunogen 
Recombinant protein encompassing a sequence within the center region of human Fibrinogen gamma. The exact sequence is proprietary.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ICC/IFIHC-PIPWB
 
*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity

HumanMouseRat
Predicted Reactivity Pig, BovinePig (84%), Bovine (83%)
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
FGG antibody
fibrinogen
gamma polypeptide antibody
fibrinogen gamma chain antibody
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Molecular Weight(MW) 52 kDa (note) (2)
Cellular Localization Secreted

Application

  • Application

    Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, ａnd the membrane was blotted with Fibrinogen gamma antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  • Application

    Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, ａnd the membrane was blotted with Fibrinogen gamma antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  • Application

    Fibrinogen gamma antibody detects Fibrinogen gamma protein by western blot analysis. Human plasma ａnd HepG2 conditioned medium were separated by 10% SDS-PAGE, ａnd the membrane was blotted with Fibrinogen gamma antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  • Application

    Fibrinogen gamma antibody detects FGG protein by western blot analysis. A. 30 µg HepG2 whole cell lysate/extract 10% SDS-PAGE Fibrinogen gamma antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  • Application

    Fibrinogen gamma antibody detects FGG protein at cytosol on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. Fibrinogen gamma antibody dilution: 1:250.

  • Application

    Fibrinogen gamma antibody detects Fibrinogen gamma protein at cylasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fibrinogen gamma protein stained by Fibrinogen gamma antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.

  • Application

    Immunoprecipitation of Fibrinogen gamma protein from HepG2 whole cell extracts using 5 μg of Fibrinogen gamma antibody . Western blot analysis was performed using Fibrinogen gamma antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Positive Control HepG2 , human plasma , HepG2 sup. , mouse plasma , rat plasma
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher. :

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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