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技術(shù)文章

FGG [C7-H9] Catalog Number：OM627729

點(diǎn)擊次數(shù)：28 發(fā)布時(shí)間：2024/9/19 11:54:39
 

Product Profile

Product Name FGG [C7-H9]
Antibody Type Primary Antibodies
Product description 
The plasma glycoprotein Fibrinogen is synthesized in the liver ａnd comprises three structurally different subunits: a, b ａnd g. Fibrinogen is important in platelet aggregation, the final step of the coagulation cascade ａnd determination of plasma viscosity ａnd erythrocyte aggregation. It is both constitutively expressed ａnd inducible during an acute phase reaction. Hemostasis following tissue injury deploys essential plasma procoagulants, which are involved in a blood coagulation cascade leading to the formation of insoluble Fibrin clots ａnd the promotion of platelet aggregation. Following vascular injury, Fibrinogen is cleaved by Thrombin to form Fibrin, which is the most abundant component of blood clots. The cleavage products of Fibrinogen regulate cell adhesion ａnd spreading, display vasoconstrictor ａnd chemotactic activities, ａnd are mitogens for several cell types.
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Immunogen 
Recombinant protein

Key Feature

Clonality Monoclonal
Isotype IgG2a
Host Species Mouse
Tested Applications

WBICCIHCFC
 
WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:100-1:500
FC:1:100-1:200
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Species Reactivity

Human
Concentration 2 mg/mL.

Target Information

Alternative Names
fggy antibody FGGY carbohydrate kinase domain containing antibody FGGY carbohydrate kinase domain-containing protein antibody FGGY_HUMAN antibody FLJ10986 antibody MGC94804 antibody OTTHUMP00000010078 antibody OTTHUMP00000010081 antibody OTTHUMP00000010082 antibody OTTHUMP00000202071 antibody RP11-242B9.1 antibody
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Molecular Weight(MW) 52 kDa
Cellular Localization Secreted.

Database Links

SwissProt ID P02679

Application

  • Application

    Fig1: Western blot analysis of FGG on human FGG recombinant protein using anti-FGG antibody at 1/1,000 dilution.

  • Application

    Fig2: ICC staining FGG (green) ａnd actin filaments (red) in 3T3-L1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using anti-FGG antibody. Counter stained with hematoxylin.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-FGG antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Flow cytometric analysis of HepG2 cells with FGG antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).

Positive Control Human FGG recombinant protein, 3T3-L1, human cerebellum tissue, human liver cancer tissue, HepG2.
Application Notes
WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:100-1:500
FC:1:100-1:200
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Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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