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Moesin antibody , C-term Catalog Number：OM263676

點擊次數(shù)：24 發(fā)布時間：2024/9/18 14:24:19
 

Product Profile

Product Name Moesin antibody , C-term
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to Moesin (moesin)Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin ａnd radixin. ERM proteins appear to function as cross-linkers between plasma membranes ａnd actin-based cytoskeletons. Moesin is localized to filopodia ａnd other membranous protrusions that are important for cell-cell recognition ａnd signaling ａnd for cell movement. [provided by RefSeq]
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Immunogen 
Recombinant protein encompassing a sequence within the C-terminus region of human Moesin. The exact sequence is proprietary.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ICC/IFIHC-PIPWB
 
*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity

HumanMouseRat
Predicted Reactivity Pig, Rhesus Monkey, BovinePig (98%), Rhesus Monkey (100%), Bovine (99%)
Concentration 0.2 mg/ml
Purification Affinity purified

Target Information

Gene Synonyms
MSN antibody
membrane-organizing extension spike protein antibody
moesin antibody
Molecular Weight(MW) 68 kDa (note)
Cellular Localization Cell membrane; Peripheral membrane protein; Cylasmic side (By similarity) , Cylasm , cytoskeleton (By similarity) , Apical cell membrane; Peripheral membrane protein; Cylasmic side (By similarity) , Cell projection , microvillus membrane; Periphe

Application

  • Application

    Moesin antibody [C2C3], C-term detects MSN protein by Western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg C8D30 whole cell lysate/extract C. 30 µg NIH-3T3 whole cell lysate/extract D. 30 µg BCL-1 whole cell lysate/extract E. 30 µg Raw264.7 whole cell lysate/extract F. 30 µg C2C12 whole cell lysate/extract 7.5 % SDS-PAGE Moesin antibody [C2C3], C-term dilution: 1:1000

  • Application

    Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE diluted at 1:1000

  • Application

    Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using Moesin antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (red) at 1:500.

  • Application

    Moesin antibody [C2C3], C-term detects Moesin protein at cell membrane in rat spleen by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. Moesin antibody [C2C3], C-term diluted at 1:500.

  • Application

    Immunoprecipitation of Moesin protein from A431 whole cell extracts using 5 μg of Moesin antibody [C2C3], C-term . Western blot analysis was performed using Moesin antibody [C2C3], C-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Positive Control NT2D1 , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher. :

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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