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技術文章

RPA1 Antibody Catalog Number：OM290268

點擊次數(shù)：25 發(fā)布時間：2024/9/18 13:16:28
 

Product Profile

Product Name RPA1 Antibody
Antibody Type Primary Antibodies
Product description 
RPA1 plays an essential role in several cellular processes in DNA metabolism including replication, recombination ａnd DNA repair. Binds ａnd subsequently stabilizes single-stranded DNA intermediates ａnd thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA ａnd probably plays a role in DNA repair; it does not support chromosomal DNA replication ａnd cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA ａnd replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair ａnd RAD51-dependent strand exchange.1) Choi, J.H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(31):13660-13665(2010)
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Immunogen 
This RPA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-559 amino acids from the C-terminal region of human RPA1.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WB
 
For WB starting dilution is: 1:1000:
Species Reactivity

Human
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol RPA1
Alternative Names
Replication protein A 70 kDa DNA-binding subunit
RP-A p70
Replication factor A protein 1
RF-A protein 1
Single-stranded DNA-binding protein
Replication protein A 70 kDa DNA-binding subunit
N-terminally processed
RPA1
REPA1
RPA70
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Molecular Weight(MW) 68 kDa

Database Links

Entrez Gene 6117
Protein Accession P27694

Application

  • Application

    Western blot analysis in K562 cell line lysates (35ug/lane).

Application Notes
For WB starting dilution is: 1:1000:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
 

原創(chuàng)作者：上海篤瑪生物科技有限公司

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