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技術文章

Phospho-Gab1-Y627 Antibody Catalog Number：OM217322

點擊次數(shù)：26 發(fā)布時間：2024/9/14 13:46:46
 

Product Profile

Product Name Phospho-Gab1-Y627 Antibody
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

DBIHC
 
DB~~1:500 IHC~~1:50~100:
Species Reactivity

Human
Concentration 1 mg/ml
Purification  

Target Information

Alternative Names
GAB1
GRB2-associated-binding protein 1
GRB2-associated binder 1
Growth factor receptor bound protein 2-associated protein 1
More 
Molecular Weight(MW) 76616 Da
Function
Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) ａnd the insulin receptor (INSR)
Tissue Specificity This Phospho-Gab1-Y627 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y627 of human GAB1.

Database Links

Entrez Gene 2549

Application

  • Application

    Dot blot analysis of anti-Phospho-Gab1-Y627 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml.

  • Application

    Formalin-fixed ａnd paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Application Notes
DB~~1:500 IHC~~1:50~100:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control ａnd phosphorylated peptides. The phospho-specific antibody is eluted with high ａnd low pH buffers ａnd neutralized immediately, followed by dialysis against PBS.
 

原創(chuàng)作者：上海篤瑪生物科技有限公司

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