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技術(shù)文章

Gab1 (phospho Tyr627) Antibody Catalog Number：OM279348

點(diǎn)擊次數(shù)：26 發(fā)布時(shí)間：2024/9/13 14:05:25
 

Product Profile

Product Name Gab1 (phospho Tyr627) Antibody
Antibody Type Primary Antibodies
Product description 
The protein encoded by Gab1 is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis ａnd plays a central role in cellular growth response, transformation ａnd apoptosis. Two transcript variants encoding different isoforms have been found for this gene.1) Anders Kallin, et al. (2004) J. Biol. Chem ; 279: 17897 - 17904. Hideto Kameda, et al. (2001) Cell Growth Differ ; 12: 307. Masaki Osawa, et al. (2002) J. Cell Biol ; 158: 773.
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Immunogen 
Gab1 (Phospho-Tyr627) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 627 (V-E-Y (p) -L-D) derived from Human Gab1.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IFWB
 
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200:
Species Reactivity

HumanMouse
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol GAB1
Alternative Names
ADDA
Growth factor receptor bound protein 2-associated protein 1
Molecular Weight(MW) 110 kDa
Tissue Specificity This antibody detects endogenous level of Gab1 only when phosphorylated at tyrosine 627.

Database Links

Entrez Gene 2549
Protein Accession NP_002030.2

Application

  • Application

    Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF using Gab1 (Phospho-Tyr627).

  • Application

    Immunofluorescence staining of methanol-fixed HeLa cells using Gab1(Phospho-Tyr627).

Application Notes
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200:

Additional Information

Form Liquid
Storage Instructions Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ ａnd Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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