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技術(shù)文章

EIF4B Antibody (C-term) Catalog Number:OM232040

點擊次數(shù):17 發(fā)布時間:2024/9/13 13:54:21
 

Product Profile

Product Name EIF4B Antibody (C-term)
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WBIHC

 
WB~~1:50~100 IHC~~1:50-100:
Species Reactivity

Human

Concentration 1 mg/ml
Purification  

Target Information

Alternative Names
EIF4B
Eukaryotic translation initiation factor 4B
Molecular Weight(MW) 69151 Da
Function
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F
Tissue Specificity This EIF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 553~583 amino acids from the C-terminal region of human EIF4B Antibody.

Database Links

Entrez Gene 1975
Nucleotide Accession Q8WYK5

Application

  • Application

    Western blot analysis of anti-EIF4B Antibody (C-term) Pab in K562 cell line lysates (35ug/lane). EIF4B(arrow) was detected using the purified Pab (1:60 dilution).

  • Application

    Western blot analysis of anti-EIF4B Antibody (C-term) Pab in 293 cell line lysates transiently transfected with the EIF4B gene (2ug/lane). EIF4B(arrow) was detected using the purified Pab (1:60 dilution).

  • Application

    Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4B Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Application Notes
WB~~1:50~100 IHC~~1:50-100:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
 

原創(chuàng)作者:上海篤瑪生物科技有限公司

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