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技術(shù)文章

eIF4B (phospho Ser422) Antibody Catalog Number：OM277787

點(diǎn)擊次數(shù)：22 發(fā)布時(shí)間：2024/9/13 13:46:28
 

Product Profile

Product Name eIF4B (phospho Ser422) Antibody
Antibody Type Primary Antibodies
Product description 
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F ａnd EIF4-A. Binds near the 5＇-terminal cap of mRNA in presence of EIF-4F ａnd ATP. Promotes the ATPase activity ａnd the ATP-dependent RNA unwinding activity of both EIF4-A ａnd EIF4-F.1) Gingras, A. C. et al. (2001) Genes Dev. 15, 807-826. Duncan, R. ａnd Hershey, J. W. (1985) J. Biol. Chem. 260, 5493-5497. Duncan, R. F. ａnd Hershey, J. W. (1989) J. Cell Biol. 109, 1467-1481.
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Immunogen 
eIF4B (phospho-Ser422) antibody was raised against a peptide sequence around phosphorylation site of Serine 422 (T-G-S (p) -E-S) derived from Human eIF4B.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHCWB
 
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

HumanMouse
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol EIF4B
Alternative Names
EIF-4B
PRO1843
ESR
NR3A1
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Molecular Weight(MW) 80 kDa
Tissue Specificity This antibody detects endogenous level of eIF4B only when phosphorylated at Serine 422.

Database Links

Entrez Gene 1975
Protein Accession NP_001408.2

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human lung carcinomatissue using eIF4B (Phospho-Ser422).

  • Application

    Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF using eIF4B (phospho-Ser422).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
Storage Instructions Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ ａnd Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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