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EIF4B [D7-F5] Catalog Number：OM627752

點(diǎn)擊次數(shù)：21 發(fā)布時(shí)間：2024/9/13 13:45:35
 

Product Profile

Product Name EIF4B [D7-F5]
Antibody Type Primary Antibodies
Product description 
The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors ａnd RNA molecules. These interactions are facilitated, in part, by the eukaryotic initiation factor 4 family (eIF4) of proteins that are involved in the early initiation of protein synthesis. eIF4B (eukaryotic translation initiation factor 4B) is a 611 amino acid protein that contains one RNA recognition motif ａnd belongs to the eIF4 protein family. Required for proper mRNA/ribosome binding, eIF4B associates with other eIF4 proteins, such as eIF4A, ａnd promotes the ATP-dependent unwinding activity of ｓelect eukaryotic initiation factors. The gene encoding eIF4B maps to human chromosome 12, which encodes over 1,100 genes ａnd comprises approximately 4.5% of the human genome.
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Immunogen 
Recombinant protein

Key Feature

Clonality Monoclonal
Isotype IgG2b
Host Species Mouse
Tested Applications

WBICCIHCFC
 
WB:1:500-1:2,000
ICC:1:100-1:500
IHC:1:100-1:500
FC:1:100-1:200
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Species Reactivity

HumanMouse
Concentration 2 mg/mL.

Target Information

Alternative Names
2310046H11Rik antibody AL024095 antibody C85189 antibody EIF 4B antibody eIF-4B antibody EIF4B antibody Eukaryotic initiation factor 4B antibody Eukaryotic translation initiation factor 4B antibody IF4B_HUMAN antibody PRO1843 antibody
Molecular Weight(MW) 80 kDa
Cellular Localization Cytosol.

Database Links

SwissProt ID P23588
Q8BGD9

Application

  • Application

    Fig1: Western blot analysis of EIF4B on human EIF4B recombinant protein using anti-EIF4B antibody at 1/1,000 dilution.

  • Application

     

  • Application

    Fig3: ICC staining EIF4B (green) ａnd actin filaments (red) in NIH-3T3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded colon cancer tissue using anti-EIF4B antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Flow cytometric analysis of Hela cells with EIF4B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

Positive Control Human EIF4B recombinant protein, A431, A549, HepG2, HEK293, HeLa, Jurkat, K562, NIH-3T3, MCF-7, colon cancer tissue.
Application Notes
WB:1:500-1:2,000
ICC:1:100-1:500
IHC:1:100-1:500
FC:1:100-1:200
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Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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