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技術文章

eIF4B (phospho Ser422) antibody Catalog Number：OM251211

點擊次數：18 發(fā)布時間：2024/9/13 13:42:49
 

Product Profile

Product Name eIF4B (phospho Ser422) antibody
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to eIF4B (Phospho Ser422)Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F ａnd EIF4-A. Binds near the 5＇-terminal cap of mRNA in presence of EIF-4F ａnd ATP. Promotes the ATPase activity ａnd the ATP-dependent RNA unwinding activity of both EIF4-A ａnd EIF4-F.
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Immunogen 
Peptide sequence around phosphorylation site of Serine 422 (T-G-S(p)-E-S) derived from Human eIF4B.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHC-PWB
 
Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity

HumanMouse
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
EIF-4B
PRO1843
EIF4B
eukaryotic translation initiation factor 4B
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Target eIF4B (Phospho Ser422)
Molecular Weight(MW) 80 (note)

Application

  • Application

    Western blot analysis of extracts from Hela cells untreated or treated with EGF using eIF4B (phospho-Ser422) Antibody

  • Application

    Immunohistochemical analysis of paraffin-embedded human lung carcinomatissue using eIF4B (Phospho-Ser422) Antibody (left) or the same antibody preincubated with blocking peptide (right).

  • Application

    Western blot analysis of extracts from Hela cells untreated or treated with EGF using eIF4B (phospho-Ser422) Antibody .

Application Notes
Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer saline (without Mg2+ ａnd Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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