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技術(shù)文章

eIF4B antibody Catalog Number：OM251212

點擊次數(shù)：20 發(fā)布時間：2024/9/13 13:42:05
 

Product Profile

Product Name eIF4B antibody
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to eIF4BRequired for the binding of mRNA to ribosomes. Functions in close association with EIF4-F ａnd EIF4-A. Binds near the 5＇-terminal cap of mRNA in presence of EIF-4F ａnd ATP. Promotes the ATPase activity ａnd the ATP-dependent RNA unwinding activity of both EIF4-A ａnd EIF4-F.
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Immunogen 
Peptide sequence around aa.420~424 (T-G-S-E-S) derived from Human eIF4B.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHC-PWB
 
Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity

Human
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
EIF-4B
PRO1843
EIF4B
eukaryotic translation initiation factor 4B
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Target eIF4B
Molecular Weight(MW) 80 (note)

Application

  • Application

    Western blot analysis of extracts from 3T3 ａnd HL60 cells using eIF4B(Ab-422) Antibody

  • Application

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4B Antibody (left) or the same antibody preincubated with blocking peptide (right).

Application Notes
Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer saline (without Mg2+ ａnd Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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