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技術(shù)文章

EIF4B Antibody Catalog Number：OM277792

點(diǎn)擊次數(shù)：21 發(fā)布時(shí)間：2024/9/13 13:37:17
 

Product Profile

Product Name EIF4B Antibody
Antibody Type Primary Antibodies
Product description 
EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F ａnd EIF4-A by binding near the 5＇- terminal cap of mRNA in presence of EIF-4F ａnd ATP. This gene also promotes the ATPase activity ａnd the ATP-dependent RNA unwinding activity of both EIF4-A ａnd EIF4-F
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Immunogen 
This EIF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-382 amino acids from the Central region of human EIF4B.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

IHC-PWB
 
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:
Species Reactivity

Human
Concentration 1 mg/ml

Target Information

Gene Symbol EIF4B
Alternative Names
Eukaryotic translation initiation factor 4B
eIF-4B
EIF4B
Molecular Weight(MW) 69 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Mouse

Database Links

Entrez Gene 1975
Protein Accession P23588

Application

  • Application

    Formalin-fixed ａnd paraffin-embedded human breast carcinoma reacted with EIF4B antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Application

    Western blot analysis of anti-EIF4B Antibody Pab in HepG2 cell line lysates (35ug/lane).

  • Application

    Western blot analysis of anti-EIF4B Antibody Pab in 293 cell line lysates transiently transfected with the EIF4B gene (2ug/lane).

  • Application

    Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.

Application Notes
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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