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技術(shù)文章

PPM1A antibody Catalog Number：OM269350

點擊次數(shù)：30 發(fā)布時間：2024/9/11 13:59:48
 

Product Profile

Product Name PPM1A antibody
Antibody Type Primary Antibodies
Product description 
Mouse monoclonal antibody [p6c7] to PPM1AThe protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, ａnd negatively regulates the activities of, MAP kinases ａnd MAP kinase kinases. It has been shown to inhibit the activation of p38 ａnd JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, ａnd thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest ａnd apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
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Immunogen 
Recombinant human PP2C alpha (1-382aa) purified from E. coli

Key Feature

Clonality Monoclonal
Isotype IgG2
Clone Number p6c7
Host Species Mouse
Tested Applications

ELISAIPWB
 
The antibody has been tested by ELISA ａnd Western blot analysis to assure specificity ａnd reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250 ~ 500. Recommended starting dilution is 1:250.:
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Species Reactivity

HumanMouse
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
PP2CA
PPM1A
protein phosphatase
Mg2+/Mn2+ dependent
1A
PP2C-ALPHA
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Target PPM1A
Light Chain Type kappa

Application

  • Application

    WB analysis of mouse liver cytosol extract using PP2C alpha antibody at a dilution of 1:250.

Application Notes
The antibody has been tested by ELISA ａnd Western blot analysis to assure specificity ａnd reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250 ~ 500. Recommended starting dilution is 1:250.:
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Additional Information

Form Liquid
Storage Instructions Can be stored at +4C. For long term storage, aliquot ａnd store at -20C. Avoid repeated freezing ａnd thawing cycles.
Storage Buffer Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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