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技術(shù)文章

INCENP antibody Catalog Number：OM259340

點擊次數(shù)：25 發(fā)布時間：2024/9/10 11:15:19
 

Product Profile

Product Name INCENP antibody
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to INCENPIn mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins ａnd ＇passenger,＇ or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), ａnd CENPD (MIM 117142). The term ＇passenger proteins＇ encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw ａnd Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cylasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); ａnd CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw ａnd Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).[supplied by OMIM, Mar 2008]
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Immunogen 
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human INCENP. The exact sequence is proprietary.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WB
 
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity

HumanMouseRat
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Synonyms
inner centromere protein
Target INCENP
Tissue Specificity Recognizes endogenous levels of INCENP protein.

Application

  • Application

    Western blot analysis of INCENP expression in Jurkat (A); Molt4 (B); SP2/0 (C); PC12 (D) whole cell lysates.

Application Notes
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form Liquid
Storage Instructions Shipped at 4 C. Upon delivery aliquot ａnd store at -20 C for one year. Avoid freeze/thaw cycles.
Storage Buffer Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; ａnd 0.01% sodium azide.
 

原創(chuàng)作者：上海篤瑪生物科技有限公司

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