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技術(shù)文章

INCENP antibody Catalog Number:OM259341

點擊次數(shù):24 發(fā)布時間:2024/9/10 11:14:02
 

Product Profile

Product Name INCENP antibody
Antibody Type Primary Antibodies
Product description
Mouse Monoclonal Antibody [3D2] to INCENPIn mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cylasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle pro
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Immunogen
The immunogen corresponding to a region within amino acids 369 and 583 of human INCENP expressed in E. Coli.

Key Feature

Clonality Monoclonal
Isotype IgG1
Clone Number 3D2
Host Species Mouse
Tested Applications

ELISAFACSICC/IFIHCIHC-PWB

 
ELISA: 1/10000.:
Species Reactivity

Human

Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification  

Target Information

Target INCENP
Molecular Weight(MW) 105.4 kDa (note)

Application

  • Application

    ELISA analysis of control antigen and antigen using INCENP antibody [3D2].

  • Application

    FACS analysis of Jurkat cells using INCENP antibody [3D2] (green) or a negative control (red).

  • Application

    ICC/IF analysis of HepG2 cells using INCENP antibody [3D2] (green) and DRAQ5 fluorescent DNA dye (blue).

  • Application

    IHC-P analysis of cervical cancer tissues using INCENP antibody [3D2].

  • Application

    IHC-P analysis of rectum cancer tissues using INCENP antibody [3D2].

  • Application

    WB analysis of lysates from (1) untransfected and (2) INCENP (a.a. 369-583)-hIgGFc transfected HEK293 cells using INCENP antibody [3D2].

  • Application

    WB analysis of human INCENP recombinant protein using INCENP antibody [3D2].

Application Notes
ELISA: 1/10000.:

Additional Information

Form Liquid
Storage Instructions 4°C; -20°C for long term storage
Storage Buffer Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer.

原創(chuàng)作者:上海篤瑪生物科技有限公司

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